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Protocol for analysis of RNA-sequencing and proteome profiling data for subgroup identification and comparison
- Source :
- STAR Protocols, Vol 3, Iss 2, Pp 101283- (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier, 2022.
-
Abstract
- Summary: RNA-sequencing and quantitative proteomic profiling simultaneously measure thousands of molecules and provide opportunities to decipher the transcriptomic and proteomic landscapes of cohort specimens for basic and health research. We present a protocol for the analysis of paired transcriptome and proteome data to identify and compare molecular subgroups among cohort specimens. We demonstrate a streamlined analysis workflow, applicable for both transcriptome and proteome data, which allows the comparison of two data types for RNA-protein variations and for derivation of biological implications.For complete details on the use and execution of this protocol, please refer to Yang et al. (2021).
- Subjects :
- Bioinformatics
Cancer
RNAseq
Gene Expression
Proteomics
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 3
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4fe8dce9ce3b43f1832816ec18e1139e
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2022.101283