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Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors
- Source :
- Virology Journal, Vol 8, Iss 1, p 40 (2011)
- Publication Year :
- 2011
- Publisher :
- BMC, 2011.
-
Abstract
- Abstract Recombinant Towne CMV expressing luciferase under the control of CMV-DNA polymerase (POL) or the late pp28 (UL99) promoters were evaluated for potential application in high-throughput screening of anti-viral compounds. POL-and pp28-luciferase displayed maximal expression 48 and 72 hours post infection, respectively. The pp28-luciferase virus achieved a wider dynamic range of luciferase expression (6-7 logs) and was selected for testing of inhibition by five anti-viral compounds. Luciferase expression highly correlated with plaque reduction and real-time PCR. The pp28-luciferase reporter system is rapid, reproducible, and highly sensitive. It may be applied to screening of novel anti-CMV compounds.
- Subjects :
- Infectious and parasitic diseases
RC109-216
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 8
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4fce68f06fa447a9900416b8a0eff43d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1743-422X-8-40