Ca2+ mediates transcription factor PuDof2.5 and suppresses stone cell production in pear fruits

Stone cells are sclerenchyma cells formed by deposition of lignin, which is the most significant factor limiting the quality of pears. Ca2+ was known to inhibit stone cells in pear fruits, but the underlying molecular mechanism remains unclear. Our study revealed that exogenous CaCl2 (Ca2+) treatment of “Nanguo” pear (Pyrus ussuriensis) suppressed the synthesis of lignin and stone cell production. We further analysed the transcriptomes using RNA-seq, identified a transcription factor, PuDof2.5, and its targets gene PuPRX42-like (lignin polymerase gene) expression decreased in CaCl2-treated samples, which are involved in suppressing lignin biosynthesis in pear fruit. PuDof2.5 was found to bind directly to the PuPRX42-like promoter and induced its transcription. Taken together, our results revealed that Ca2+ modulated the key lignin biosynthetic transcription factor PuDof2.5 to suppress stone cell production in pear fruits.