Genome-wide analysis of DNA methylation in bronchial washings

Authors :: Sang-Won Um
Yujin Kim
Bo Bin Lee
Dongho Kim
Kyung-Jong Lee
Hong Kwan Kim
Joungho Han
Hojoong Kim
Young Mog Shim
Duk-Hwan Kim
Source :: Clinical Epigenetics, Vol 10, Iss 1, Pp 1-9 (2018)
Publication Year :: 2018
Publisher :: BMC, 2018.
Abstract: Abstract Background The objective of this study was to discover DNA methylation biomarkers for detecting non-small lung cancer (NSCLC) in bronchial washings and understanding the association between DNA methylation and smoking cessation. Methods DNA methylation was analyzed in bronchial washing samples from 70 NSCLCs and 53 hospital-based controls using Illumina HumanMethylation450K BeadChip. Methylation levels in these bronchial washings were compared to those in 897 primary lung tissues of The Cancer Genome Atlas (TCGA) data. Results Twenty-four CpGs (p