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Subcellular analysis of blood-brain barrier function by micro-impalement of vessels in acute brain slices

Authors :
Amira Sayed Hanafy
Pia Steinlein
Julika Pitsch
Mariella Hurtado Silva
Natascha Vana
Albert J. Becker
Mark Evan Graham
Susanne Schoch
Alf Lamprecht
Dirk Dietrich
Source :
Nature Communications, Vol 14, Iss 1, Pp 1-19 (2023)
Publication Year :
2023
Publisher :
Nature Portfolio, 2023.

Abstract

The blood-brain barrier (BBB) tightly and dynamically regulates exchange with the brain. Here, the authors report a brain slice approach to study the native BBB in a highly controlled manner by combining intravascular perfusion and multiphoton microscopy.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.4cb633c9c9004c6bbbdb5c97f9e673c4
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-023-36070-6