Back to Search
Start Over
Isolation of a calcium-binding peptide from tilapia scale protein hydrolysate and its calcium bioavailability in rats
- Source :
- Journal of Functional Foods, Vol 6, Iss , Pp 575-584 (2014)
- Publication Year :
- 2014
- Publisher :
- Elsevier, 2014.
-
Abstract
- A calcium-binding peptide was successfully purified from tilapia (Oreochromis niloticus) scale protein hydrolysate by hydroxylapatite affinity (HA), gel filtration and reverse phase high-performance liquid chromatography (RP-HPLC). This peptide was sequenced as DGDDGEAGKIG (N- to C-terminal, MW = 1033 Da) based on MALDI-TOF-MS/MS. There was no significant difference in body weight gain, serum P content or alkaline phosphatase (ALP) level in Ca-deficient rats after supplementation with TSPH–Ca (tilapia scale protein hydrolysate calcium complex), CPP–Ca (casein phosphopeptide calcium complex), CaCO3 or saline (control). However, the apparent absorptivity of calcium, serum and femur Ca content, femur bone mineral density and strength in the rats fed with TSPH–Ca were significantly higher than those of rats in the control and CaCO3 groups (P 0.05), indicating that TSPH possesses the potential to serve as an alternative for CPP.
Details
- Language :
- English
- ISSN :
- 17564646
- Volume :
- 6
- Issue :
- 575-584
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Functional Foods
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4c507c3214ae4ae19b892a55501b07a7
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.jff.2013.12.001