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Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC)

Authors :
Soriano Francisco
Ponte Carmen
Blanco Miguel
Blanco Jesus E
Aldeguer Xavier
Blanco Jorge
Naves Plínio
Martinez-Medina Margarita
Darfeuille-Michaud Arlette
Garcia-Gil L Jesus
Source :
BMC Microbiology, Vol 9, Iss 1, p 202 (2009)
Publication Year :
2009
Publisher :
BMC, 2009.

Abstract

Abstract Background Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a feature of certain pathogenic E. coli strains, we compared the biofilm formation capacity of 27 AIEC and 38 non-AIEC strains isolated from the intestinal mucosa. Biofilm formation capacity was then contrasted with the AIEC phenotype, the serotype, the phylotype, and the presence of virulence genes. Results Specific biofilm formation (SBF) indices were higher amongst AIEC than non-AIEC strains (P = 0.012). In addition, 65.4% of moderate to strong biofilms producers were AIEC, whereas 74.4% of weak biofilm producers were non-AIEC (P = 0.002). These data indicate that AIEC strains were more efficient biofilm producers than non-AIEC strains. Moreover, adhesion (P = 0.009) and invasion (P = 0.003) indices correlated positively with higher SBF indices. Additionally, motility (100%, P < 0.001), H1 type flagellin (53.8%, P < 0.001), serogroups O83 (19.2%, P = 0.008) and O22 (26.9%, P = 0.001), the presence of virulence genes such as sfa/focDE (38.5%, P = 0.003) and ibeA (26.9%, P = 0.017), and B2 phylotype (80.8%, P < 0.001) were frequent characteristics amongst biofilm producers. Conclusion The principal contribution of the present work is the finding that biofilm formation capacity is a novel, complementary pathogenic feature of the recently described AIEC pathovar. Characterization of AIEC specific genetic determinants, and the regulatory pathways, involved in biofilm formation will likely bring new insights into AIEC pathogenesis.

Subjects

Subjects :
Microbiology
QR1-502

Details

Language :
English
ISSN :
14712180
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Microbiology
Publication Type :
Academic Journal
Accession number :
edsdoj.4c49866c812940df9361184d5a434c76
Document Type :
article
Full Text :
https://doi.org/10.1186/1471-2180-9-202