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In Silico Identification of Dysregulated miRNAs Targeting KRAS Gene in Pancreatic Cancer

Authors :
Asbiel Felipe Garibaldi-Ríos
Luis E. Figuera
Guillermo Moisés Zúñiga-González
Belinda Claudia Gómez-Meda
Patricia Montserrat García-Verdín
Irving Alejandro Carrillo-Dávila
Itzae Adonai Gutiérrez-Hurtado
Blanca Miriam Torres-Mendoza
Martha Patricia Gallegos-Arreola
Source :
Diseases, Vol 12, Iss 7, p 152 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Pancreatic cancer (PC) is highly lethal, with KRAS mutations in up to 95% of cases. miRNAs inversely correlate with KRAS expression, indicating potential as biomarkers. This study identified miRNAs targeting KRAS and their impact on PC characteristics using in silico methods. dbDEMC identified dysregulated miRNAs in PC; TargetScan, miRDB, and PolymiRTS 3.0 identified miRNAs specific for the KRAS gene; and OncomiR evaluated the association of miRNAs with clinical characteristics and survival in PC. The correlation between miRNAs and KRAS was analysed using ENCORI/starBase. A total of 210 deregulated miRNAs were identified in PC (116 overexpressed and 94 underexpressed). In total, 16 of them were involved in the regulation of KRAS expression and 9 of these (hsa-miR-222-3p, hsa-miR-30a-5p, hsa-miR-30b-5p, hsa-miR-30e-5p, hsa-miR-377-3p, hsa-miR-495-3p, hsa-miR-654-3p, hsa-miR-877-5p and hsa-miR-885-5p) were associated with the clinical characteristics of the PC. Specifically, the overexpression of hsa-miR-30a-5p was associated with PC mortality, and hsa-miR-30b-5p, hsa-miR-377-3p, hsa-miR-495-3p, and hsa-miR-885-5p were associated with survival. Correlation analysis revealed that the expression of 10 miRNAs is correlated with KRAS expression. The dysregulated miRNAs identified in PC may regulate KRAS and some are associated with clinically relevant features, highlighting their potential as biomarkers and therapeutic targets in PC treatment. However, experimental validation is required for confirmation.

Details

Language :
English
ISSN :
20799721
Volume :
12
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Diseases
Publication Type :
Academic Journal
Accession number :
edsdoj.4bc820cbb4684daea2b74bbeff049cec
Document Type :
article
Full Text :
https://doi.org/10.3390/diseases12070152