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Measurement of HDL-Cholesterol in Serum by New Enzymatic Method
- Source :
- Siriraj Medical Journal, Vol 53, Iss 7 (2020)
- Publication Year :
- 2020
- Publisher :
- Faculty of Medicine Siriraj Hospital, 2020.
-
Abstract
- High density lipoprotein (HDL) are responsible for the reverse transport of cholesterol from the peripheral cells to the liver. Here, cholesterol is metabolised. Monitoring of HDL-C in serum is of clinical importance since an inverse correlation exists between serum HDL-C concentrations and the risk of atherosclerotic disease. Elevated HDL-C concentrations are protective against coronary heart disease. In this study, an automated enzymatic method for the direct determination of HDL-C in serum was found to have good precision and accuracy. Reproducibility was determined daily using control Precinorm L® in an internal protocol (n=20), within-run CV = 1.3% and between-day CV = 2.013%. The comparison of the HDL-C enzymatic assay with HDL-C precipitating agent was good with a correlation coefficient r = 0.965, Y = 1.981+ 0.959X, n =172. The normal range of HDL-C concentration in serum by this enzymatic method was 36.16 -86.08 mg/dl. Thus, a HDL-C level of over 35 mg/dl, shows the risk is not high and is protective against coronary heart disease.
- Subjects :
- Measurement
HDL-Cholesterol
Serum
New Enzymatic
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 22288082
- Volume :
- 53
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Siriraj Medical Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4bb1bb53cba849348b6a68762926e41a
- Document Type :
- article