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AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis

Authors :
Hong Jie Zhu
Bo Zhang
Wanqing Wei
Shuang He Liu
Lang Xiang
Jiapeng Zhu
Rui Hua Jiao
Yasuhiro Igarashi
Ghader Bashiri
Yong Liang
Ren Xiang Tan
Hui Ming Ge
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

Macrocyclization is an important process in bioactive natural product synthesis. Here, the authors report on the study of a macrocyclic ring constructing enzyme in the biosynthesis of alchivemycin A and using gene deletion, biochemical assays and isotope labelling show the enzyme catalyses tandem dehydration and Michael-type addition.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.4b5a7495b704b4bbee8ca1abd98b0d5
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-32088-4