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UFObow: A single-wavelength excitable Brainbow for simultaneous multicolor ex-vivo and in-vivo imaging of mammalian cells

Authors :
Jiahong Hu
Fangfang Yang
Chong Liu
Nengzhi Wang
Yinghan Xiao
Yujie Zhai
Xinru Wang
Ren Zhang
Lulu Gao
Mengli Xu
Jialu Wang
Zheng Liu
Songlin Huang
Wenfeng Liu
Yajing Hu
Feng Liu
Yuqi Guo
Liang Wang
Jing Yuan
Zhihong Zhang
Jun Chu
Source :
Communications Biology, Vol 7, Iss 1, Pp 1-13 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract Brainbow is a genetic cell-labeling technique that allows random colorization of multiple cells and real-time visualization of cell fate within a tissue, providing valuable insights into understanding complex biological processes. However, fluorescent proteins (FPs) in Brainbow have distinct excitation spectra with peak difference greater than 35 nm, which requires sequential imaging under multiple excitations and thus leads to long acquisition times. In addition, they are not easily used together with other fluorophores due to severe spectral bleed-through. Here, we report the development of a single-wavelength excitable Brainbow, UFObow, incorporating three newly developed blue-excitable FPs. We have demonstrated that UFObow enables not only tracking the growth dynamics of tumor cells in vivo but also mapping spatial distribution of immune cells within a sub-cubic centimeter tissue, revealing cell heterogeneity. This provides a powerful means to explore complex biology in a simultaneous imaging manner at a single-cell resolution in organs or in vivo.

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
23993642
Volume :
7
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Communications Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.4b50f54377480f9c356784d3fda04e
Document Type :
article
Full Text :
https://doi.org/10.1038/s42003-024-06062-3