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RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)

Authors :
Wenyi Zhang
Pengchao Wang
Yiwei Xiong
Tianyong Chen
Sufei Jiang
Hui Qiao
Yongsheng Gong
Yan Wu
Shubo Jin
Hongtuo Fu
Source :
Genes, Vol 13, Iss 11, p 2079 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Cyclin B (CycB) plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (Macrobrachium nipponense). A phylo-genetic tree analysis revealed that the protein sequence of Mn-CycB was most closely related to those of freshwater prawns, whereas the evolutionary distance from crabs was much longer. A quantitative PCR analysis showed that the expression of Mn-CycB was highest in the gonad of both male and female prawns compared to that in other tissues (p < 0.05), indicating that this gene may play essential roles in the regulation of both testis and ovary development in M. nipponense. In males, Mn-CycB expression in the testis and androgenic gland was higher during the reproductive season than during the non-reproductive season (p < 0.05), implying that CycB plays essential roles in the reproductive development of male M. nipponense. An RNA interference analysis revealed that the Mn-insulin-like androgenic gland hormone expression decreased as the Mn-CycB expression decreased, and that few sperm were detected 14 days after the dsCycB treatment, indicating that CycB positively affects testis development in M. nipponense. The results of this study highlight the functions of CycB in M. nipponense, and they can be applied to studies of male reproductive development in other crustacean species.

Details

Language :
English
ISSN :
20734425
Volume :
13
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Genes
Publication Type :
Academic Journal
Accession number :
edsdoj.4ae839c390604603980723730ec4b813
Document Type :
article
Full Text :
https://doi.org/10.3390/genes13112079