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Decreased uncoupling protein 2 expression in aging retinal pigment epithelial cells
- Source :
- International Journal of Ophthalmology, Vol 12, Iss 3, Pp 375-380 (2019)
- Publication Year :
- 2019
- Publisher :
- Press of International Journal of Ophthalmology (IJO PRESS), 2019.
-
Abstract
- AIM: To analyze the expression of uncoupling protein 2 (UCP2) in retinal pigment epithelium (RPE) cells at the different human age, further explore the possible new target of RPE cells protection. METHODS: Adult retinal pigment epithelial-19 (ARPE-19) cells and the primary RPE cells at the different age (9-20y, RESULTS: Cells from the donors more than 60y are larger and more fibroblastic in appearance compared to ARPE-19 cells and those primary cultures obtained from the younger individuals by using phase-contrast micrographs. Results of RT-PCR, Western blot and confocal microscopy all showed that UCP2 was highly expressed in ARPE-19 cells and in the younger primary cultured human RPE cells at the age of 9-20y and 50-55y, whereas lower expression of UCP2 was measured in the older primary cultured human RPE cells at the age more than 60y. CONCLUSION: Expression of UCP2 gene is decreased in aged RPE cells, promoting the lower ability of anti-oxidation in these cells. It is indicated that UCP2 gene might be a new target for protecting the cells from oxidative stress damage.
Details
- Language :
- English
- ISSN :
- 22223959 and 22274898
- Volume :
- 12
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- International Journal of Ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4a93cf82b14fcf8ef5b0cabab22fd5
- Document Type :
- article
- Full Text :
- https://doi.org/10.18240/ijo.2019.03.04