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Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme
- Source :
- Czech Journal of Food Sciences, Vol 32, Iss 1, Pp 48-53 (2014)
- Publication Year :
- 2014
- Publisher :
- Czech Academy of Agricultural Sciences, 2014.
-
Abstract
- The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using β-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cyclodextrin glycosyltransferase were 55°C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.
- Subjects :
- cyclodextrin glycosyltransferase
affinity chromatography
purification
Agriculture
Subjects
Details
- Language :
- English
- ISSN :
- 12121800 and 18059317
- Volume :
- 32
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Czech Journal of Food Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4a55ee6c917f4e37862cff7e7fd7f19f
- Document Type :
- article
- Full Text :
- https://doi.org/10.17221/432/2012-CJFS