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Serial small- and wide-angle X-ray scattering with laboratory sources

Authors :
Mark A. Levenstein
Karen Robertson
Thomas D. Turner
Liam Hunter
Cate O'Brien
Cedrick O'Shaughnessy
Alexander N. Kulak
Pierre Le Magueres
Jakub Wojciechowski
Oleksandr O. Mykhaylyk
Nikil Kapur
Fiona C. Meldrum
Source :
IUCrJ, Vol 9, Iss 5, Pp 538-543 (2022)
Publication Year :
2022
Publisher :
International Union of Crystallography, 2022.

Abstract

Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.

Details

Language :
English
ISSN :
20522525
Volume :
9
Issue :
5
Database :
Directory of Open Access Journals
Journal :
IUCrJ
Publication Type :
Academic Journal
Accession number :
edsdoj.4a0479f93e5743d1a1f08c93027b5fe2
Document Type :
article
Full Text :
https://doi.org/10.1107/S2052252522007631