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Serial small- and wide-angle X-ray scattering with laboratory sources
- Source :
- IUCrJ, Vol 9, Iss 5, Pp 538-543 (2022)
- Publication Year :
- 2022
- Publisher :
- International Union of Crystallography, 2022.
-
Abstract
- Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.
Details
- Language :
- English
- ISSN :
- 20522525
- Volume :
- 9
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- IUCrJ
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4a0479f93e5743d1a1f08c93027b5fe2
- Document Type :
- article
- Full Text :
- https://doi.org/10.1107/S2052252522007631