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Nano-Integrated Plant Tissue Culture to Increase the Rate of Callus Induction, Growth, and Curcuminoid Production in Curcuma longa

Authors :
Muhammad Iqbal
Zill-e-Huma Aftab
Tehmina Anjum
Humaira Rizwana
Waheed Akram
Arusa Aftab
Zahoor Ahmad Sajid
Guihua Li
Source :
Plants, Vol 13, Iss 13, p 1819 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Turmeric has attracted a significant amount of interest in recent years due to its strong antimicrobial properties. The tissue culture of turmeric is preferred to obtain disease-free, highest number of plantlets with good uniform chemistry. However, there is a need to increase the speed of the whole process to meet the growing demand for planting materials and to save time and resources. Iron oxide nanoparticles (Fe3O4 NPs) showed positive effects on callus initiation time, proliferation rate, percent root response, shoot length, percent rooting, and number of roots per explant. Highest callus induction, i.e., 80%, was recorded in cultures that were grown in the presence of 15 mg/L of Fe3O4 NPs. Callus initiated earlier in culture tubes that received green synthesized iron nanoparticles in a concentration between 10–15 mg/L. Biofabricated nanoparticles were characterized for their size, physiochemical, and optical properties through UV–Vis spectroscopy, FTIR, XRD, and SEM. Curcuminoids profiling was performed by implementing LC-Ms that revealed increased quantities in plantlets grown in nano-supplemented media when compared to the control.

Details

Language :
English
ISSN :
22237747
Volume :
13
Issue :
13
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.49ababbb09b24fc396c91b711af565e3
Document Type :
article
Full Text :
https://doi.org/10.3390/plants13131819