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Cross Cultivation on Homologous/Heterologous Plant-Based Culture Media Empowers Host-Specific and Real Time In Vitro Signature of Plant Microbiota

Authors :
Hend Elsawey
Eman H. Nour
Tarek R. Elsayed
Rahma A. Nemr
Hanan H. Youssef
Mervat A. Hamza
Mohamed Abbas
Mahmoud El-Tahan
Mohamed Fayez
Silke Ruppel
Nabil A. Hegazi
Source :
Diversity, Vol 15, Iss 1, p 46 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Alliances of microbiota with plants are masked by the inability of in vitro cultivation of their bulk. Pure cultures piled in international centers originated from dissimilar environments/hosts. Reporting that plant root/leaf-based culture media support the organ-specific growth of microbiota, it was of interest to further investigate if a plant-based medium prepared from homologous (maize) supports specific/adapted microbiota compared to another prepared from heterologous plants (sunflower). The culture-independent community of maize phyllosphere was compared to communities cross-cultivated on plant broth-based media: CFU counts and taxa prevalence (PCR-DGGE; Illumina MiSeq amplicon sequencing). Similar to total maize phyllospheric microbiota, culture-dependent communities were overwhelmed by Proteobacteria (>94.3–98.3%); followed by Firmicutes (>1.3–3.7%), Bacteroidetes (>0.01–1.58%) and Actinobacteria (>0.06–0.34%). Differential in vitro growth on homologous versus heterologous plant-media enriched/restricted various taxa. In contrast, homologous cultivation over represented members of Proteobacteria (ca. > 98.0%), mainly Pseudomonadaceae and Moraxellaceae; heterologous cultivation and R2A enriched Firmicutes (ca. > 3.0%). The present strategy simulates/fingerprints the chemical composition of host plants to expand the culturomics of plant microbiota, advance real-time in vitro cultivation and lab-keeping of compatible plant microbiota, and identify preferential pairing of plant-microbe partners toward future synthetic community (SynComs) research and use in agriculture.

Details

Language :
English
ISSN :
14242818
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Diversity
Publication Type :
Academic Journal
Accession number :
edsdoj.48c4a447ea54e97af99f2c06e89d712
Document Type :
article
Full Text :
https://doi.org/10.3390/d15010046