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Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse

Authors :
Alex Graça Contato
Tássio Brito de Oliveira
Guilherme Mauro Aranha
Emanuelle Neiverth de Freitas
Ana Claudia Vici
Karoline Maria Vieira Nogueira
Rosymar Coutinho de Lucas
Ana Sílvia de Almeida Scarcella
Marcos Silveira Buckeridge
Roberto Nascimento Silva
Maria de Lourdes Teixeira de Moraes Polizeli
Source :
Microorganisms, Vol 9, Iss 3, p 533 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.

Details

Language :
English
ISSN :
20762607
Volume :
9
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.480e6155e3a49838c05ae0bf2b03377
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms9030533