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One-step fabrication of 3D silver paste electrodes into microfluidic devices for enhanced droplet-based cell sorting

Authors :
Lang Rao
Bo Cai
Xiao-Lei Yu
Shi-Shang Guo
Wei Liu
Xing-Zhong Zhao
Source :
AIP Advances, Vol 5, Iss 5, Pp 057134-057134-9 (2015)
Publication Year :
2015
Publisher :
AIP Publishing LLC, 2015.

Abstract

3D microelectrodes are one-step fabricated into a microfluidic droplet separator by filling conductive silver paste into PDMS microchambers. The advantages of 3D silver paste electrodes in promoting droplet sorting accuracy are systematically demonstrated by theoretical calculation, numerical simulation and experimental validation. The employment of 3D electrodes also helps to decrease the droplet sorting voltage, guaranteeing that cells encapsulated in droplets undergo chip-based sorting processes are at better metabolic status for further potential cellular assays. At last, target droplet containing single cell are selectively sorted out from others by an appropriate electric pulse. This method provides a simple and inexpensive alternative to fabricate 3D electrodes, and it is expected our 3D electrode-integrated microfluidic droplet separator platform can be widely used in single cell operation and analysis.

Subjects

Subjects :
Physics
QC1-999

Details

Language :
English
ISSN :
21583226
Volume :
5
Issue :
5
Database :
Directory of Open Access Journals
Journal :
AIP Advances
Publication Type :
Academic Journal
Accession number :
edsdoj.47a58d6935bc4db69679481221f9b7f2
Document Type :
article
Full Text :
https://doi.org/10.1063/1.4921317