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Polysaccharides from Tumorous stem mustard prevented high fructose diet-induced non-alcoholic fatty liver disease by regulating gut microbiota, hepatic lipid metabolism, and the AKT/FOXO1/MAPK signaling pathway
- Source :
- Journal of Functional Foods, Vol 102, Iss , Pp 105448- (2023)
- Publication Year :
- 2023
- Publisher :
- Elsevier, 2023.
-
Abstract
- Ingestion of 30 % fructose water (HF) for eight weeks caused non-alcoholic fatty liver disease (NAFLD) in mice and manifested in prominent weight gain, insulin resistance, abnormal lipid metabolism, oxidative stress, and liver damage. Biochemical analysis suggested that treatment with non-starch polysaccharides extracted from Tumorous stem mustard (NSPTSM) at 25–50 mg/kg·bw prevents these symptoms in HF-induced NAFLD. 16S rRNA sequencing revealed that NSPTSM balanced the HF-perturbed colonic microbiota and significantly increased the relative abundance of Akkermansia in mice. Metabonomics results indicated that the hepatic biomarkers were LPE (18:1/0:0) and LPC (16:1/0:0), which were negatively related to cecal 23-nordeoxycholic acid. Transcriptome and western blot analysis suggested that protein kinase B (AKT)/forkhead box protein O-1 (FOXO1)/mitogen-activated protein kinase (MAPK) signaling pathway played an important role in prevention of HF-induced NAFLD by NSPTSM. These findings indicated that NSPTSM protected mice against HF-induced NAFLD and underscored the efficacy of utilizing NSPTSM as a possible prebiotic.
Details
- Language :
- English
- ISSN :
- 17564646
- Volume :
- 102
- Issue :
- 105448-
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Functional Foods
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.46dd7a841b94f539c0c3c7a1db1b698
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.jff.2023.105448