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Comparison of Surface Plasmon Resonance, Resonant Waveguide Grating Biosensing and Enzyme Linked Immunosorbent Assay (ELISA) in the Evaluation of a Dengue Virus Immunoassay

Authors :
Joe Buechler
Xiaoyan Che
Jing Jin
Catriona McElnea
Yue Chen
Yuxian Pan
Liwen Qiu
Xixia Ding
Johnny X. Huang
Scott R. Fry
Dongmei Hu
Matthew A. Cooper
Source :
Biosensors, Vol 3, Iss 3, Pp 297-311 (2013)
Publication Year :
2013
Publisher :
MDPI AG, 2013.

Abstract

Two label-free biosensor platforms, Resonance Waveguide Grating (RWG) and Surface Plasmon Resonance (SPR), were used to rank a large panel of anti-dengue virus NS1 antibodies. Dengue non-structural 1 (NS1) protein is an established serological marker for the early detection of dengue infection. A variety of commercial dengue NS1 antigen capture immunoassays are available in both ELISA and lateral flow format. However, there is a significant scope to improve both the sensitivity and the specificity of those tests. The interactions of antibody (Ab)-antigen (Ag) were profiled, with weak interactions (KD = 1–0.1 μM) able to be detected under static equilibrium conditions by RWG, but not observed to under more rigorous flow conditions using SPR. There were significant differences in the absolute affinities determined by the two technologies, and there was a poor correlation between antibodies best ranked by RWG and the lower limit of detection (LLOD) found by ELISA. Hence, whilst high-throughput RWG can be useful as preliminary screening for higher affinity antibodies, care should be exercised in the assignation of quantitative values for affinity between different assay formats.

Details

Language :
English
ISSN :
20796374
Volume :
3
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.469fc544952a4e0c90544a1e7a0a4dcc
Document Type :
article
Full Text :
https://doi.org/10.3390/bios3030297