Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization

Authors :: Dongjian Cao
Sa Wu
Caili Xi
Dong Li
Kaiheng Zhu
Zhihong Zhang
Hui Gong
Qingming Luo
Jie Yang
Source :: Communications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
Publication Year :: 2021
Publisher :: Nature Portfolio, 2021.
Abstract: Cao et al. present a method to prepare long single-strand DNA concatemers (lssDNAc) through a controllable rolling-circle amplification (CRCA), used to develop AmpFISH workflows. Their method is suitable for labelling to both individual mRNA molecules and chromosomes and can also be multiplexed with immunofluorescence, allowing for sensitive RNA/DNA detection.

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