Back to Search
Start Over
Molecular stability of a vaccine strain of Canine coronavirus after serial passages in A72 cells
- Source :
- Brazilian Journal of Veterinary Research and Animal Science, Vol 54, Iss 1 (2017)
- Publication Year :
- 2017
- Publisher :
- Universidade de São Paulo, 2017.
-
Abstract
- Canine coronavirus (CCoV) exists in types I and II and infects dogs leading mainly to enteritis, though type II has already been associated with generalized and highly lethal infection. A CCoV-type II inactivated vaccine produced in A72 canine cells is available worldwide and largely used, though the molecular stability after serial passages of vaccine seeds is unknown. This article reports the evolution of the CCoV-II vaccine strain 1-71 in A72 cells based on partial S gene sequencing, showing the predominance of neutral evolution and the occurrence of four sites under purifying selection. Thus, cell-adapted strains of CCoV-II may be genetically stable after serial passages in a same cell line due to a stable virus-host relationship.
- Subjects :
- Canine coronavirus
Vaccine
Passages
Evolution
A72 cells
Animal culture
SF1-1100
Subjects
Details
- Language :
- English, Portuguese
- ISSN :
- 16784456 and 14139596
- Volume :
- 54
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Brazilian Journal of Veterinary Research and Animal Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.461de58d83074b379cbc9f42223dfbad
- Document Type :
- article
- Full Text :
- https://doi.org/10.11606/issn.1678-4456.bjvras.2017.111310