Back to Search Start Over

Molecular stability of a vaccine strain of Canine coronavirus after serial passages in A72 cells

Authors :
Iracema Nunes de Barros
Sheila Oliveira de Souza Silva
Sueli Akemi Tanikawi
Paulo Eduardo Brandão
Source :
Brazilian Journal of Veterinary Research and Animal Science, Vol 54, Iss 1 (2017)
Publication Year :
2017
Publisher :
Universidade de São Paulo, 2017.

Abstract

Canine coronavirus (CCoV) exists in types I and II and infects dogs leading mainly to enteritis, though type II has already been associated with generalized and highly lethal infection. A CCoV-type II inactivated vaccine produced in A72 canine cells is available worldwide and largely used, though the molecular stability after serial passages of vaccine seeds is unknown. This article reports the evolution of the CCoV-II vaccine strain 1-71 in A72 cells based on partial S gene sequencing, showing the predominance of neutral evolution and the occurrence of four sites under purifying selection. Thus, cell-adapted strains of CCoV-II may be genetically stable after serial passages in a same cell line due to a stable virus-host relationship.

Details

Language :
English, Portuguese
ISSN :
16784456 and 14139596
Volume :
54
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Brazilian Journal of Veterinary Research and Animal Science
Publication Type :
Academic Journal
Accession number :
edsdoj.461de58d83074b379cbc9f42223dfbad
Document Type :
article
Full Text :
https://doi.org/10.11606/issn.1678-4456.bjvras.2017.111310