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Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells
- Source :
- BMB Reports, Vol 46, Iss 4, Pp 201-206 (2013)
- Publication Year :
- 2013
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2013.
-
Abstract
- Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is anisothiocyanate found in some cruciferous vegetables, especiallybroccoli. Sulforaphane has been shown to displayanti-cancer properties against various cancer cell lines. Matrixmetalloproteinase-9 (MMP-9), which degrades the extracellularmatrix (ECM), plays an important role in cancer cell invasion.In this study, we investigated the effect of sulforaphane on12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9expression and cell invasion in MCF-7 cells. TPA-inducedMMP-9 expression and cell invasion were decreased bysulforaphane treatment. TPA substantially increased NF-κB andAP-1 DNA binding activity. Pre-treatment with sulforaphaneinhibited TPA-stimulated NF-κB binding activity, but not AP-1binding activity. In addition, we found that sulforaphanesuppressed NF-κB activation, by inhibiting phosphorylation ofIκB in TPA-treated MCF-7 cells. In this study, we demonstratedthat the inhibition of TPA-induced MMP-9 expression and cellinvasion by sulforaphane was mediated by the suppression ofthe NF-κB pathway in MCF-7 cells. [BMB Reports 2013; 46(4):201-206]
Details
- Language :
- English
- ISSN :
- 19766696 and 1976670X
- Volume :
- 46
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- BMB Reports
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4617868b209141fdba44728686b73220
- Document Type :
- article
- Full Text :
- https://doi.org/10.5483/BMBRep.2013.46.4.160