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Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?

Authors :
Yanara Alessandra Santana Moura
Ariadne Tennyle Vieira De Souza
Páblo Eugênio Da Costa e Silva
Marllyn Marques Da Silva
Ana Lúcia Figueiredo Porto
Raquel Pedrosa Bezerra
Source :
Phycology, Vol 3, Iss 4, Pp 436-446 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Thrombosis is characterized by the pathological formation of fibrin clots within a blood vessel, leading to the obstruction of blood flow. Fibrinolytic enzymes from microorganisms have been shown to be more efficient and safer in dissolving clots. Then, this study aimed to evaluate the cell growth and fibrinolytic enzyme production of Tetradesmus obliquus under different cultivation conditions. T. obliquus grew under autotrophic and mixotrophic conditions using different concentrations of corn steep liquor (0.25 ≤ CSL ≤ 4.00%). The cells were concentrated and lysed via two different methods (sonication or homogenization) to trigger the release of the enzyme. It was precipitated via acetone or ammonium sulfate additions and purified using ion exchange chromatography. The highest biomass productivity (Px = 130 ± 12.8 mg∙L−1day−1), specific growth rate (µmax = 0.17 ± 0.00 day−1), and fibrinolytic activity (391 ± 40.0 U∙mg−1) was achieved on a mixotrophic cultivation at a 0.25% CSL concentration. The results showed that the homogenizing method had better performance in the release of enzyme, and the precipitation with acetone obtained the highest fibrinolytic activity (567 ± 49.3 U∙mg−1). The purified enzyme showed a specific activity of 1221 ± 31 U∙mg−1 and a molecular mass of 97 kDa. So, the fibrinolytic enzyme from T. obliquus had higher activity when compared to the other fibrinolytic enzymes, being a potential source for the development of therapeutic agents in thrombosis treatment. Additional studies are needed to investigate the biochemical properties and biological profile of this enzyme.

Details

Language :
English
ISSN :
26739410
Volume :
3
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Phycology
Publication Type :
Academic Journal
Accession number :
edsdoj.45b7e225bc214abaaa6d334b9f957e86
Document Type :
article
Full Text :
https://doi.org/10.3390/phycology3040029