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Fast three-color single-molecule FRET using statistical inference

Authors :
Janghyun Yoo
Jae-Yeol Kim
John M. Louis
Irina V. Gopich
Hoi Sung Chung
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.43303b5b95fe4c4c8636c35d24a12b28
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-17149-w