Back to Search Start Over

3D-Printed Tumor-on-Chip for the Culture of Colorectal Cancer Microspheres: Mass Transport Characterization and Anti-Cancer Drug Assays

Authors :
Mónica Gabriela Sánchez-Salazar
Regina Crespo-López Oliver
Sofía Ramos-Meizoso
Valeri Sofía Jerezano-Flores
Salvador Gallegos-Martínez
Edna Johana Bolívar-Monsalve
Carlos Fernando Ceballos-González
Grissel Trujillo-de Santiago
Mario Moisés Álvarez
Source :
Bioengineering, Vol 10, Iss 5, p 554 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Tumor-on-chips have become an effective resource in cancer research. However, their widespread use remains limited due to issues related to their practicality in fabrication and use. To address some of these limitations, we introduce a 3D-printed chip, which is large enough to host ~1 cm3 of tissue and fosters well-mixed conditions in the liquid niche, while still enabling the formation of the concentration profiles that occur in real tissues due to diffusive transport. We compared the mass transport performance in its rhomboidal culture chamber when empty, when filled with GelMA/alginate hydrogel microbeads, or when occupied with a monolithic piece of hydrogel with a central channel, allowing communication between the inlet and outlet. We show that our chip filled with hydrogel microspheres in the culture chamber promotes adequate mixing and enhanced distribution of culture media. In proof-of-concept pharmacological assays, we biofabricated hydrogel microspheres containing embedded Caco2 cells, which developed into microtumors. Microtumors cultured in the device developed throughout the 10-day culture showing >75% of viability. Microtumors subjected to 5-fluorouracil treatment displayed

Details

Language :
English
ISSN :
23065354
Volume :
10
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Bioengineering
Publication Type :
Academic Journal
Accession number :
edsdoj.42ea8aec3c7242898469249d2fb80f72
Document Type :
article
Full Text :
https://doi.org/10.3390/bioengineering10050554