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Crosstalk between CRISPR-Cas9 and the human transcriptome

Authors :
Aaron A. Smargon
Assael A. Madrigal
Brian A. Yee
Kevin D. Dong
Jasmine R. Mueller
Gene W. Yeo
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

The off-target effects of CRISPR-Cas9 are thought to be mediated by its cognate guide RNA. Here the authors show that Cas9 independently interacts with the human transcriptome, correlating with elevated RNA editing even under guide RNA co-expression.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.41abe7312ab41079c5067ddeabb873b
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-28719-5