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Effect of matrine on proliferation and migration of human thyroid cancer cell line TPC-1
- Source :
- Jichu yixue yu linchuang, Vol 41, Iss 12, Pp 1730-1735 (2021)
- Publication Year :
- 2021
- Publisher :
- Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2021.
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Abstract
- Objective To investigate the mechanism of matrine inhibiting the proliferation and migration of thyroid cancer cell line TPC-1 through down-regulated miRNA-182-5p TPC-1. Methods TPC-1 cells were transfected after 24 hours with 0.5 mg/mL matrine or without 0.5 mg/mL matrine, the experiments were divided into: inhibitor-NC, inhibitor against miR-182-5p (inhibitor), mimic-NC, miRNA-182-5p-mimics, mimic-NC+matrine and miR-182-5p- mimics+matrine. miR-182-5p, E-cadherin, N-cadherin and vinmentin gene were detected by PCR. TPC-1 cells proliferation and migration were detected using CCK-8 and Transwell assay. The expression of E-cadherin、N-cadherin and vinmentin protein was determined by Western blot. Results Compared with Nthy-ori3-1 (NT), miR-182-5p was highly expressed in TPC-1 cells (P<0.01); down-regulation to the expression of miRNA-182-5p inhibited the proliferation and migration of TPC-1 cells (P<0.01); Compared to the 0 mg/mL matrine group, 0.5 mg/mL matrine significantly reduced the miR-182-5p level (P<0.05) and inhibited proliferation, migration of TPC-1 cells and also reduced N-cadherin and vinmentin expression but increased E-cadherin expression in TPC-1 cells (P<0.01); Over-expression of miR-182-5p partly abolished the matrine-inhibited proliferation and migration of TPC-1 cells and the increased E-cadherin expression, decreased miR-182-5p,N-cadherin and vinmentin expression (P<0.01). Conclusions Matrine is a potential therapeutic drug for papillary thyroid carcinoma (PTC) and miR-182 may be an effective target for the treatment of PTC.
- Subjects :
- matrine
mir-182-5p
proliferation
migration
papillary thyroid cancer
Medicine
Subjects
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 41
- Issue :
- 12
- Database :
- Directory of Open Access Journals
- Journal :
- Jichu yixue yu linchuang
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4125a1de9ed48debb1f96dc92219113
- Document Type :
- article