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Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility

Authors :
Valentina Bergamini
Elisa Resca
Alberto Portone
Tiziana Petrachi
Francesco Ganzerli
Stefano Truzzi
Giorgio Mari
Luigi Rovati
Massimo Dominici
Elena Veronesi
Source :
Pharmaceutics, Vol 15, Iss 8, p 2043 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The Corning Epic® label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufacturing of blood filters, a category of medical devices representing life-saving therapies for the increasing number of patients with kidney failure. The biocompatibility assays were carried out by developing a cell model aimed at mimicking the clinical use of the blood filters and complementing the existing cytotoxicity assay requested by ISO10993-5. Experiments were performed by putting fibroblasts in both direct contact with two types of selected resins, and indirect contact by means of homemade customized well inserts that were precisely designed and developed for this technology. For both types of contact, fibroblasts were cultured in medium and human plasma. ELF tests confirmed the biocompatibility of both resins, highlighting a statistically significant different biological behavior of a polyaromatic resin compared to control and ion-exchanged resin, when materials were in indirect contact and soaking with plasma. Overall, the ELF test is able to mimic clinical scenarios and represents a promising approach to investigate biocompatibility, showing peculiar biological behaviors and suggesting the activation of specific intracellular pathways.

Details

Language :
English
ISSN :
19994923
Volume :
15
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Pharmaceutics
Publication Type :
Academic Journal
Accession number :
edsdoj.4100a4be082f4cb2a0778dd1056c1454
Document Type :
article
Full Text :
https://doi.org/10.3390/pharmaceutics15082043