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Loss of Claudin-3 Impairs Hepatic Metabolism, Biliary Barrier Function, and Cell Proliferation in the Murine LiverSummary

Authors :
Felix Alexander Baier
Daniel Sánchez-Taltavull
Tural Yarahmadov
Cristina Gómez Castellà
Fadi Jebbawi
Adrian Keogh
Riccardo Tombolini
Adolfo Odriozola
Mariana Castro Dias
Urban Deutsch
Mikio Furuse
Britta Engelhardt
Benoît Zuber
Alex Odermatt
Daniel Candinas
Deborah Stroka
Source :
Cellular and Molecular Gastroenterology and Hepatology, Vol 12, Iss 2, Pp 745-767 (2021)
Publication Year :
2021
Publisher :
Elsevier, 2021.

Abstract

Background & Aims: Tight junctions in the liver are essential to maintain the blood-biliary barrier, however, the functional contribution of individual tight junction proteins to barrier and metabolic homeostasis remains largely unexplored. Here, we describe the cell type–specific expression of tight junction genes in the murine liver, and explore the regulation and functional importance of the transmembrane protein claudin-3 in liver metabolism, barrier function, and cell proliferation. Methods: The cell type–specific expression of hepatic tight junction genes is described using our mouse liver single-cell sequencing data set. Differential gene expression in Cldn3-/- and Cldn3+/+ livers was assessed in young and aged mice by RNA sequencing (RNA-seq), and hepatic tissue was analyzed for lipid content and bile acid composition. A surgical model of partial hepatectomy was used to induce liver cell proliferation. Results: Claudin-3 is a highly expressed tight junction protein found in the liver and is expressed predominantly in hepatocytes and cholangiocytes. The histology of Cldn3-/- livers showed no overt phenotype, and the canalicular tight junctions appeared intact. Nevertheless, by RNA-seq we detected a down-regulation of metabolic pathways in the livers of Cldn3-/- young and aged mice, as well as a decrease in lipid content and a weakened biliary barrier for primary bile acids, such as taurocholic acid, taurochenodeoxycholic acid, and taurine-conjugated muricholic acid. Coinciding with defects in the biliary barrier and lower lipid metabolism, there was a diminished hepatocyte proliferative response in Cldn3-/- mice after partial hepatectomy. Conclusions: Our data show that, in the liver, claudin-3 is necessary to maintain metabolic homeostasis, retention of bile acids, and optimal hepatocyte proliferation during liver regeneration. The RNA-seq data set can be accessed at: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE159914.

Details

Language :
English
ISSN :
2352345X
Volume :
12
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Cellular and Molecular Gastroenterology and Hepatology
Publication Type :
Academic Journal
Accession number :
edsdoj.40c8e3eb1f4103a6858cde3ba57550
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jcmgh.2021.04.003