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Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE

Authors :
Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade
Source :
Electronic Journal of Plant Breeding, Vol 6, Iss 1, Pp 264-268 (2015)
Publication Year :
2015
Publisher :
Indian Society of Plant Breeders, 2015.

Abstract

In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.

Details

Language :
English
ISSN :
0975928X
Volume :
6
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Electronic Journal of Plant Breeding
Publication Type :
Academic Journal
Accession number :
edsdoj.40ab45532d848b59b993eaa910dbdd4
Document Type :
article