Back to Search
Start Over
Co-Incubation of Human Spermatozoa with Anti-VDAC Antibody Reduced Sperm Motility
- Source :
- Cellular Physiology and Biochemistry, Vol 33, Iss 1, Pp 142-150 (2014)
- Publication Year :
- 2014
- Publisher :
- Cell Physiol Biochem Press GmbH & Co KG, 2014.
-
Abstract
- Background: Voltage-dependent anion channel (VDAC), a channel protein, exists in the outer mitochondrial membrane of somatic cells and is involved in multiple physiological and pathophysiological processes. Up until now, little has been known about VDAC in male germ cells. In the present study, the relationship between VDAC and human sperm motility was explored. Methods: Highly motile human spermatozoa were incubated in vitro with anti-VDAC antibody. Total sperm motility, straight line velocity (VSL), curvilinear velocity (VCL), and average path velocity (VAP) were recorded. Intracellular free calcium concentration ([Ca2+]i), pH value (pHi), and ATP content were determined. Results: Co-incubation with anti-VDAC antibody reduced VSL, VCL, and VAP of spermatozoa. Co-incubation further reduced [Ca2+]i. Anti-VDAC antibody did not significantly alter total sperm motility, pHi and intracellular ATP content. Conclusion: The data suggest that co-incubation with anti-VDAC antibody reduces sperm motility through inhibition of Ca2+ transmembrane flow. In this way, VDAC participates in the modulation of human sperm motility through mediating Ca2+ transmembrane transport and exchange.
- Subjects :
- Human
Sperm motility
Calcium
pH
ATP
VDAC
Physiology
QP1-981
Biochemistry
QD415-436
Subjects
Details
- Language :
- English
- ISSN :
- 10158987 and 14219778
- Volume :
- 33
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Cellular Physiology and Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3fc07c4f8b6b477e9f0c0004041c5369
- Document Type :
- article
- Full Text :
- https://doi.org/10.1159/000356657