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Antibacterial evaluation of different prosthetic liner textiles coated by CuO nanoparticles

Authors :
Ziba Najmi
Nives Matijaković Mlinarić
Alessandro Calogero Scalia
Andrea Cochis
Atiđa Selmani
Aleksander Učakar
Anže Abram
Anamarija Zore
Ida Delač
Ivan Jerman
Nigel Van de Velde
Janja Vidmar
Klemen Bohinc
Lia Rimondini
Source :
Heliyon, Vol 10, Iss 1, Pp e23849- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Prosthetic liners are mainly used as an interface between residual limbs and prosthetic sockets to minimize physical and biological damage to soft tissue. However, the closed and moist conditions within liners and the amputee's skin provide a suitable environment for bacterial growth to cause infections. This study aimed to coat a comprehensive variant material with copper oxide nanoparticles (CuO NPs) and compare their surface analysis and antibacterial properties. These materials were covered with CuO NPs solution at a concentration of 70 μg mL−1 to achieve this purpose. After drying, their surface characteristics were analyzed by measuring zeta potential, contact angle, surface roughness, and fiber arrangement. Cu-released concentration from the coatings into the acetate buffer solution by inductively coupled plasma mass spectrometry indicated that lycra and nylon quickly released Cu ions to concentrations up to ∼0.2 μg mL−1 after 24 h, causing low metabolic activity of human bone–marrow mesenchymal stem cells (bMSC) in the indirect assay. Antibacterial activity of the coated specimens was evaluated by infecting their surfaces with the Gram-positive bacteria Staphylococcus epidermidis, reporting a significant ∼40 % reduction of metabolic activity for x-dry after 24 h; in addition, the number of viable bacterial colonies adhered to the surface of this material was reduced by ∼23 times in comparison with non-treated x-dry that were visually confirmed by scanning electron microscope. In conclusion, CuO NPs x-dry shows optimistic results to pursue further experiments due to its slow speed of Cu release and prolonged antibacterial activity, as well as its compatibility with human cells.

Details

Language :
English
ISSN :
24058440
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Heliyon
Publication Type :
Academic Journal
Accession number :
edsdoj.3f794594ae9c412fb48670287284c8e8
Document Type :
article
Full Text :
https://doi.org/10.1016/j.heliyon.2023.e23849