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In vitro Antigen-presentation Assay for Self- and Microbial-derived Antigens

Authors :
Laura Campisi
Source :
Bio-Protocol, Vol 7, Iss 11 (2017)
Publication Year :
2017
Publisher :
Bio-protocol LLC, 2017.

Abstract

Antigen presenting cells (APC) are able to process and present to T cells antigens from different origins. This mechanism is highly regulated, in particular by Patter Recognition Receptor (PRR) signals. Here, I detail a protocol designed to assess in vitro the capacity of APC to present antigens derived from bacteria, apoptotic and infected apoptotic cells.

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
23318325
Volume :
7
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Bio-Protocol
Publication Type :
Academic Journal
Accession number :
edsdoj.3f783fc983df47d7bef8ce9dffa7906d
Document Type :
article
Full Text :
https://doi.org/10.21769/BioProtoc.2307