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Enhanced production of cordycepin in Ophiocordyceps sinensis using growth supplements under submerged conditions

Enhanced production of cordycepin in Ophiocordyceps sinensis using growth supplements under submerged conditions

Authors :
Vikas Kaushik
Amanvir Singh
Aditi Arya
Sangeeta Chahal Sindhu
Anil Sindhu
Ajay Singh
Source :
Biotechnology Reports, Vol 28, Iss , Pp e00557- (2020)
Publication Year :
2020
Publisher :
Elsevier, 2020.

Abstract

Cordycepin is a crucial bioactive compound produced by the fungus Cordyceps spp. Its therapeutic potential has been recognized for a wide range of biological properties such as anticancer, anti-diabetic, antidepressant, antioxidant, immunomodulation, etc. Moreover, its human random clinical trials depicted a promising anti-inflammatory activity that reduced the airway inflammation remarkably in asthmatic patients. But its overexploitation and low production of cordycepin in naturally growing biomass are insufficient to meet its existing market demand for its therapeutic use. Therefore, strategies for enhancement of cordycepin production in Cordyceps spp. are warranted. However, specifically, wild type Ophiocordyceps sinensis possesses a very low content of cordycepin and has restricted growth in natural mycelial biomass. To overcome these limitations, this study attempted to enhance cordycepin production in its mycelial biomass in vitro under submerged conditions by adding various growth supplements. The effect of these growth supplements was evaluated by reversed-phase high-performance liquid chromatography (RP-HPLC) which demonstrated that among nucleosides- hypoxanthine and adenosine; amino acids-glycine and glutamine; plant hormones- 1-naphthaleneacetic acid (NAA) and 3-indoleacetic acid (IAA); vitamin-thiamine (B1) from each group of growth supplements yielded a higher amount of cordycepin with 466.48 ± 3.88, 380.23 ± 1.78, 434.97 ± 2.32, 269.78 ± 2.92, 227.61 ± 2.34, 226.02 ± 1.69 and 185.26 ± 2.35 mg/L respectively as compared to control with 13.66 ± 0.64 mg/L. Further, at the transcriptional level, quantitative real time-polymerase chain reaction (qRT-PCR) analysis of genes associated with metabolism and cordycepin biosynthesis depicted significant upregulation of major downstream genes- NT5E, RNR, purA, and ADEK which corroborated well with RP-HPLC analysis. Taken together, the present study identified growth supplements as potential precursors to activate the cordycepin biosynthesis pathway leading to improved cordycepin production in O. sinensis.

Details

Language :
English
ISSN :
2215017X
Volume :
28
Issue :
e00557-
Database :
Directory of Open Access Journals
Journal :
Biotechnology Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.3f170b77468a49688af35b8c5ad846e7
Document Type :
article
Full Text :
https://doi.org/10.1016/j.btre.2020.e00557