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Cytokinin-Regulated Expression of Arabidopsis thaliana PAP Genes and Its Implication for the Expression of Chloroplast-Encoded Genes

Authors :
Aleksandra A. Andreeva
Radomira Vankova
Ivan A. Bychkov
Natalia V. Kudryakova
Maria N. Danilova
Jozef Lacek
Elena S. Pojidaeva
Victor V. Kusnetsov
Source :
Biomolecules, Vol 10, Iss 12, p 1658 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Cytokinins (CKs) are known to regulate the biogenesis of chloroplasts under changing environmental conditions and at different stages of plant ontogenesis. However, the underlying mechanisms are still poorly understood. Apparently, the mechanisms can be duplicated in several ways, including the influence of nuclear genes that determine the expression of plastome through the two-component CK regulatory circuit. In this study, we evaluated the role of cytokinins and CK signaling pathway on the expression of nuclear genes for plastid RNA polymerase-associated proteins (PAPs). Cytokinin induced the expression of all twelve Arabidopsis thalianaPAP genes irrespective of their functions via canonical CK signaling pathway but this regulation might be indirect taking into consideration their different functions and versatile structure of promoter regions. The disruption of PAP genes contributed to the abolishment of positive CK effect on the accumulation of the chloroplast gene transcripts and transcripts of the nuclear genes for plastid transcription machinery as can be judged from the analysis of pap1 and pap6 mutants. However, the CK regulatory circuit in the mutants remained practically unperturbed. Knock-out of PAP genes resulted in cytokinin overproduction as a consequence of the strong up-regulation of the genes for CK synthesis.

Details

Language :
English
ISSN :
2218273X and 03934756
Volume :
10
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Biomolecules
Publication Type :
Academic Journal
Accession number :
edsdoj.3f03934756e2401e8f8e9be4944e6ff1
Document Type :
article
Full Text :
https://doi.org/10.3390/biom10121658