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Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Authors :
Manuel C. Martos-Maldonado
Christian T. Hjuler
Kasper K. Sørensen
Mikkel B. Thygesen
Jakob E. Rasmussen
Klaus Villadsen
Søren R. Midtgaard
Stefan Kol
Sanne Schoffelen
Knud J. Jensen
Source :
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
Publication Year :
2018
Publisher :
Nature Portfolio, 2018.

Abstract

His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.3e78d4a3733a4cc3b314ad992f024827
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-018-05695-3