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Chitlac-Coated Thermosets Enhance Osteogenesis and Angiogenesis in a Co-culture of Dental Pulp Stem Cells and Endothelial Cells

Authors :
Monica Rapino
Valentina Di Valerio
Susi Zara
Marialucia Gallorini
Guya D. Marconi
Silvia Sancilio
Eleonora Marsich
Barbara Ghinassi
Viviana di Giacomo
Amelia Cataldi
Source :
Nanomaterials, Vol 9, Iss 7, p 928 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Dental pulp stem cells (DPSCs) represent a population of stem cells which could be useful in oral and maxillofacial reconstruction. They are part of the periendothelial niche, where their crosstalk with endothelial cells is crucial in the cellular response to biomaterials used for dental restorations. DPSCs and the endothelial cell line EA.hy926 were co-cultured in the presence of Chitlac-coated thermosets in culture conditions inducing, in turn, osteogenic or angiogenic differentiation. Cell proliferation was evaluated by 3‒[4,5‒dimethyl‒thiazol‒2‒yl‒]‒2,5‒diphenyl tetrazolium bromide (MTT) assay. DPSC differentiation was assessed by measuring Alkaline Phosphtase (ALP) activity and Alizarin Red S staining, while the formation of new vessels was monitored by optical microscopy. The IL-6 and PGE2 production was evaluated as well. When cultured together, the proliferation is increased, as is the DPSC osteogenic differentiation and EA.hy926 vessel formation. The presence of thermosets appears either not to disturb the system balance or even to improve the osteogenic and angiogenic differentiation. Chitlac-coated thermosets confirm their biocompatibility in the present co-culture model, being capable of improving the differentiation of both cell types. Furthermore, the assessed co-culture appears to be a useful tool to investigate cell response toward newly synthesized or commercially available biomaterials, as well as to evaluate their engraftment potential in restorative dentistry.

Details

Language :
English
ISSN :
20794991
Volume :
9
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Nanomaterials
Publication Type :
Academic Journal
Accession number :
edsdoj.3e06803fb1cc492e87c65d466bcb7ac0
Document Type :
article
Full Text :
https://doi.org/10.3390/nano9070928