Back to Search Start Over

Comparison of Modified Celsior Solution and M-Kyoto Solution for Pancreas Preservation in Human Islet Isolation

Authors :
Hirofumi Noguchi M.D., Ph.D.
Bashoo Naziruddin
Nicholas Onaca
Andrew Jackson
Masayuki Shimoda
Tetsuya Ikemoto
Yasutaka Fujita
Naoya Kobayashi
Marlon F. Levy
Shinichi Matsumoto M.D., Ph.D.
Source :
Cell Transplantation, Vol 19 (2010)
Publication Year :
2010
Publisher :
SAGE Publishing, 2010.

Abstract

Since the successful demonstration of the Edmonton protocol, islet transplantation has advanced significantly on several fronts, including improved pancreas preservation systems. In this study, we evaluated two different types of organ preservation solutions for human islet isolation. Modified Celsior (Celsior solution with hydroxyethyl starch and nafamostat mesilate; HNC) solution and modified Kyoto (MK) solution were compared for pancreas preservation prior to islet isolation. Islet yield after purification was significantly higher in the MK group than in the HNC group (MK = 6186 ± 985 IE/g; HNC = 3091 ± 344 IE/g). The HNC group had a longer phase I period (digestion time), a higher volume of undigested tissue, and a higher percentage of embedded islets, suggesting that the solution may inhibit collagenase. However, there was no significant difference in ATP content in the pancreata or in the attainability of posttransplant normoglycemia in diabetic nude mice between the two groups, suggesting that the quality of islets was similar among the two groups. In conclusion, MK solution is better for pancreas preservation before islet isolation than HNC solution due to the higher percentage of islets that can be isolated from the donor pancreas. MK solution should be the solution of choice among the commercially available solutions for pancreatic islet isolation leading to transplantation.

Subjects

Subjects :
Medicine

Details

Language :
English
ISSN :
09636897 and 15553892
Volume :
19
Database :
Directory of Open Access Journals
Journal :
Cell Transplantation
Publication Type :
Academic Journal
Accession number :
edsdoj.3d7b289b4aae4df89fb475ad65c7d9be
Document Type :
article
Full Text :
https://doi.org/10.3727/096368909X508852