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HtrA1 serine protease expression levels on age-related macular degeneration (AMD) patients in Yogyakarta

Authors :
Supanji Supanji
Bahana Ilham Perdamaian Ayudha
Dianratri Anindita
Syifarahmah Anditta
Wahyu Widayanti Tri
Setya Wardhana Firman
Bayu Sasongko Muhammad
Eko Prayogo Mohammad
Nurini Agni Angela
Oka Chio
Source :
BIO Web of Conferences, Vol 28, p 02004 (2020)
Publication Year :
2020
Publisher :
EDP Sciences, 2020.

Abstract

This research aims to investigate the HtrA1 serine protease circulating level of Age-related Macular Degeneration (AMD) patients in Yogyakarta, Indonesia. This study was conducted from January to August 2019 which included 38 AMD patients and 16 Non-AMD patients/controls (two groups). Baseline data and blood sample were collected. ELISA assay was used to measure the HtrA1 serine protease circulating level on both groups. SNP genotyping of rs10490924 was using restriction enzyme digestion. This study used The IBM SPSS® version 24 (Chicago, The USA) to determine the relationship between HtrA1 expression level and AMD incidence. AMD patients had higher HtrA1 serine protease level (35.31) than controls (30.08). However, there is no association found between HtrA1 serine protease level and AMD incidence (p-value>0.05, CI 95 %). However, HtrA1 serine protease did not associate positively to AMD incidence in Yogyakarta samples. Further analysis by grouping AMD patient based on the rs10490924 genotype show no statistical correlation between HTRA1 to the incidence of AMD. This result might be due to the lack of samples in the study groups. Future studies with larger number of samples are advised to better see the association between Htra1 serine protease level and AMD incidence.

Details

Language :
English, French
ISSN :
21174458
Volume :
28
Database :
Directory of Open Access Journals
Journal :
BIO Web of Conferences
Publication Type :
Academic Journal
Accession number :
edsdoj.3cf4d382fbc480dac983fb6e1228ab5
Document Type :
article
Full Text :
https://doi.org/10.1051/bioconf/20202802004