Back to Search Start Over

Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis

Authors :
Mark Gerstein
Erol Fikrig
Sebastian Kurscheid
Hongyu Zhao
Xiaomei Wang
Zhong Wang
Roger P. Alexander
Vincent Bruno
Wei Zheng
Lisa Chung
Feng Qian
Michael Snyder
Ruth R. Montgomery
Source :
Viruses, Vol 5, Iss 7, Pp 1664-1681 (2013)
Publication Year :
2013
Publisher :
MDPI AG, 2013.

Abstract

The West Nile virus (WNV) is an emerging infection of biodefense concern and there are no available treatments or vaccines. Here we used a high-throughput method based on a novel gene expression analysis, RNA-Seq, to give a global picture of differential gene expression by primary human macrophages of 10 healthy donors infected in vitro with WNV. From a total of 28 million reads per sample, we identified 1,514 transcripts that were differentially expressed after infection. Both predicted and novel gene changes were detected, as were gene isoforms, and while many of the genes were expressed by all donors, some were unique. Knock-down of genes not previously known to be associated with WNV resistance identified their critical role in control of viral infection. Our study distinguishes both common gene pathways as well as novel cellular responses. Such analyses will be valuable for translational studies of susceptible and resistant individuals—and for targeting therapeutics—in multiple biological settings.

Details

Language :
English
ISSN :
19994915
Volume :
5
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.3c92f3430fb8481fbb4782f52ece1d03
Document Type :
article
Full Text :
https://doi.org/10.3390/v5071664