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Determination of insecticides’ lethal concentrations and metabolic enzyme levels in Triatoma dimidiata
- Source :
- Salud Pública de México, Vol 62, Iss 4, jul-ago, Pp 402-409 (2020)
- Publication Year :
- 2020
- Publisher :
- Instituto Nacional de Salud Pública, 2020.
-
Abstract
- Objective. The feasibility of the use of WHO impregnated paper and biochemical assays to determine lethal concentra­tions (LC50 and LC99) and insecticide metabolic enzyme levels of Triatoma dimidiata. Materials and methods. LC50 and LC99 were calculated with WHO papers impregnated at differ­ent concentrations of malathion, propoxur and deltamethrin; the percentage of insensitive acetylcholinesterase (iAChE); and the levels of esterases, glutathione S-transferases, and monooxygenases in laboratory nymphs of the first stage (5 to 7 days), were undertaken using the WHO biochemical assays. Results. Respectively the LC50 and LC99 μg/cm2 obtained for malathion were 43.83 and 114.38, propoxur 4.71 and 19.29, and deltamethrin 5.80 and 40.46. A 30% of the population had an iAChE, and only a few individuals had high P450 and β-eterase levels. Conclusion. Impregnated papers and bio­chemical tests developed by WHO for other insects, proved to be feasible methods in monitoring insecticide resistance and metabolic enzymes involved in T. dimidiata.
Details
- Language :
- English, Spanish; Castilian
- ISSN :
- 00363634
- Volume :
- 62
- Issue :
- 4, jul-ago
- Database :
- Directory of Open Access Journals
- Journal :
- Salud Pública de México
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3c676a4d5ecc4331b48ac567b3dd6f66
- Document Type :
- article
- Full Text :
- https://doi.org/10.21149/10133