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The Association between Gut Microbiota and Serum Biomarkers in Children with Atopic Dermatitis

Authors :
Irina G. Kalashnikova
Alexandra I. Nekrasova
Anna V. Korobeynikova
Maria M. Bobrova
German A. Ashniev
Sirozhdin Yu. Bakoev
Angelica V. Zagainova
Mariya V. Lukashina
Larisa R. Tolkacheva
Ekaterina S. Petryaikina
Alexander S. Nekrasov
Sergey I. Mitrofanov
Tatyana A. Shpakova
Lidiya V. Frolova
Natalya V. Bulanova
Ekaterina A. Snigir
Vladimir E. Mukhin
Vladimir S. Yudin
Valentin V. Makarov
Anton A. Keskinov
Sergey M. Yudin
Source :
Biomedicines, Vol 12, Iss 10, p 2351 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Background. Currently, it is known that the gut microbiota plays an important role in the functioning of the immune system, and a rebalancing of the bacterial community can arouse complex immune reactions and lead to immune-mediated responses in an organism, in particular, the development of atopic dermatitis (AD). Cytokines and chemokines are regulators of the innate and adaptive immune response and represent the most important biomarkers of the immune system. It is known that changes in cytokine profiles are a hallmark of many diseases, including atopy. However, it remains unclear how the bacterial imbalance disrupts the function of the immune response in AD. Objectives. We attempted to determine the role of gut bacteria in modulating cytokine pathways and their role in atopic inflammation. Methods. We sequenced the 16S rRNA gene from 50 stool samples of children aged 3–12 years who had confirmed atopic dermatitis, and 50 samples from healthy children to serve as a control group. To evaluate the immune status, we conducted a multiplex immunofluorescence assay and measured the levels of 41 cytokines and chemokines in the serum of all participants. Results. To find out whether changes in the composition of the gut microbiota were significantly associated with changes in the level of inflammatory cytokines, a correlation was calculated between each pair of bacterial family and cytokine. In the AD group, 191 correlations were significant (Spearman’s correlation coefficient, p ≤ 0.05), 85 of which were positive and 106 which were negative. Conclusions. It has been demonstrated that intestinal dysbiosis is associated with alterations in cytokine profiles, specifically an increase in proinflammatory cytokine concentrations. This may indicate a systemic impact of these conditions, leading to an imbalance in the immune system’s response to the Th2 type. As a result, atopic conditions may develop. Additionally, a correlation between known AD biomarkers (IL-5, IL-8, IL-13, CCL22, IFN-γ, TNF-α) and alterations in the abundance of bacterial families (Pasteurellaceae, Barnesiellaceae, Eubacteriaceae) was observed.

Details

Language :
English
ISSN :
22279059
Volume :
12
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Biomedicines
Publication Type :
Academic Journal
Accession number :
edsdoj.3b53fab0390d48efbb5c7e9ee9609cf9
Document Type :
article
Full Text :
https://doi.org/10.3390/biomedicines12102351