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A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.
- Source :
- PLoS Biology, Vol 20, Iss 9, p e3001772 (2022)
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 15449173 and 15457885
- Volume :
- 20
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- PLoS Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3acdec6eadb747dba6da2bd4ef33714b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1371/journal.pbio.3001772