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Metagenomic Sequencing to Analyze Composition and Function of Top-Gray Chalky Grain Microorganisms from Hybrid Rice Seeds

Authors :
You Liu
Yuan Yuan
Hui Yuan
Yan Wang
Chenzhong Jin
Hao Zhang
Jianliang Tang
Yihong Hu
Source :
Plants, Vol 12, Iss 12, p 2358 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The top-gray chalkiness of hybrid rice (Oryza sativa L.) seeds is a typical phenomenon in hybrid rice seeds. The chalky part of the grain is infected and is the inoculum to infect the normal seeds during storage and soaking. These seed-associated microorganisms were cultivated and sequenced using metagenomics shotgun sequencing to obtain more comprehensive information on the seed-associated microorganisms in this experiment. The results showed that fungi could grow well on the rice flour medium, similar to the ingredients of rice seed endosperms. After the assembly of metagenomic data, a gene catalog was established, comprising 250,918 genes. Function analysis showed that glycoside hydrolases were the dominant enzymes, and the genus Rhizopus accounted for the dominant microorganisms. The fungal species R. microspores, R. delemar, and R. oryzae were likely to be the candidate pathogens in the top-gray chalky grains of hybrid rice seeds. These results will provide a reference for improving hybrid rice processing after harvest.

Details

Language :
English
ISSN :
22237747
Volume :
12
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.3aacd05087994dfdb27b65968926c8e8
Document Type :
article
Full Text :
https://doi.org/10.3390/plants12122358