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A Brief Review of Molecular Techniques to Assess Plant Diversity

Authors :
Ibrahim A. Arif
Mohammad A. Bakir
Haseeb A. Khan
Ahmad H. Al Farhan
Ali A. Al Homaidan
Ali H. Bahkali
Mohammad Al Sadoon
Mohammad Shobrak
Source :
International Journal of Molecular Sciences, Vol 11, Iss 5, Pp 2079-2096 (2010)
Publication Year :
2010
Publisher :
MDPI AG, 2010.

Abstract

Massive loss of valuable plant species in the past centuries and its adverse impact on environmental and socioeconomic values has triggered the conservation of plant resources. Appropriate identification and characterization of plant materials is essential for the successful conservation of plant resources and to ensure their sustainable use. Molecular tools developed in the past few years provide easy, less laborious means for assigning known and unknown plant taxa. These techniques answer many new evolutionary and taxonomic questions, which were not previously possible with only phenotypic methods. Molecular techniques such as DNA barcoding, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), microsatellites and single nucleotide polymorphisms (SNP) have recently been used for plant diversity studies. Each technique has its own advantages and limitations. These techniques differ in their resolving power to detect genetic differences, type of data they generate and their applicability to particular taxonomic levels. This review presents a basic description of different molecular techniques that can be utilized for DNA fingerprinting and molecular diversity analysis of plant species.

Details

Language :
English
ISSN :
14220067
Volume :
11
Issue :
5
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.3a4ac1f2dd3b491d8712e11d6ef5f314
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms11052079