Back to Search
Start Over
Enrichment and identification of Δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
- Source :
- Data in Brief, Vol 4, Iss C, Pp 641-649 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier, 2015.
-
Abstract
- This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.
Details
- Language :
- English
- ISSN :
- 23523409
- Volume :
- 4
- Issue :
- C
- Database :
- Directory of Open Access Journals
- Journal :
- Data in Brief
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.398e4a5b20b847dfa0c3e415f10b09d0
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.dib.2015.07.033