Back to Search Start Over

Quality and intensity of the tissue response to two synthetic granular hydroxyapatite implanted in critical defects of rat calvaria

Authors :
Neusa Motta de Freitas Costa
Bruno Raposo Melo
Rosana Tavares Brito
Gustavo Vicentis de Oliveira Fernandes
Vagner Gonçalves Bernardo
Eliene Carvalho Fonseca
Marcio Baltazar Conz
Glória Almeida Soares
José Mauro Granjeiro
Source :
Materials Research, Vol 12, Iss 2, Pp 245-251 (2009)
Publication Year :
2009
Publisher :
Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol), 2009.

Abstract

The objective of the present study was to evaluate the quality and intensity of the tissue response to two synthetic hydroxyapatites implanted in critical defects in the skulls of rats. Sixty animals were divided into three experimental groups: I (control), II (HA-1 = HA with 28% crystallinity) and III (HA-2 = HA with 70% crystallinity). They were sacrificed 1, 3, 6, and 9 months after implantation (n = 5 individuals per group/period). Histomorphometric analysis included i) counting of polymorphonuclear leucocytes, mast cells, macrophages and foreign body multinucleated giant cells stained with anti-lysozyme; ii) microvascular density stained with anti-Factor VIII and iii) degree of cell proliferation stained with anti-PCNA. There were no significant differences between the experimental groups in either the quality or quantity of cells in the inflammatory infiltrate, or the degree of angiogenesis and cell proliferation. We conclude that HA-1 and HA-2 are biocompatible and that the physico-chemical differences of these biomaterials did not affect cellular response.

Details

Language :
English
ISSN :
15161439
Volume :
12
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Materials Research
Publication Type :
Academic Journal
Accession number :
edsdoj.38a16fb14a5a4c09b56811719047b70d
Document Type :
article
Full Text :
https://doi.org/10.1590/S1516-14392009000200022