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Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae

Authors :
Podolska Agnieszka
Anthon Christian
Bak Mads
Tommerup Niels
Skovgaard Kerstin
Heegaard Peter MH
Gorodkin Jan
Cirera Susanna
Fredholm Merete
Source :
BMC Genomics, Vol 13, Iss 1, p 459 (2012)
Publication Year :
2012
Publisher :
BMC, 2012.

Abstract

Abstract Background MicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus pleuropneumoniae (APP) causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is still very limited. Results In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were significantly up-regulated in the necrotic sample and 12 were down-regulated. The expression analysis of a number of candidates revealed microRNAs of potential importance in the innate immune response. MiR-155, a known key player in inflammation, was found expressed in both samples. Moreover, miR-664-5p, miR-451 and miR-15a appear as very promising candidates for microRNAs involved in response to pathogen infection. Conclusions This is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.

Details

Language :
English
ISSN :
14712164
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Genomics
Publication Type :
Academic Journal
Accession number :
edsdoj.3891e6d84b04f97966bb191ecb63ad9
Document Type :
article
Full Text :
https://doi.org/10.1186/1471-2164-13-459